Phospho s536
WebHuman Phospho-RelA/NFkB p65 (S536) Antibody Summary Species Reactivity Human Specificity Detects human RelA/NF kappa B p65 when phosphorylated at S536. Source … WebJun 8, 2024 · p-RELA/NFκB p65 Antibody (27.Ser 536) is a mouse monoclonal IgG 1 κ, cited in 255 publications, provided at 200 µg/ml raised against a short amino acid sequence containing phosphorylated Ser 536 of NFκB p65 of human origin recommended for detection of Ser 536 phosphorylated NFκB p65 of mouse, rat and human origin by WB, IP, IF, IHC (P) …
Phospho s536
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WebSer 536 is phosphorylated by IKKbeta while Ser 529 by CKII. In TNFalpha induction pSer536 but not 529 is required for p65 activation at least in some cell systems. Cite 7 … WebEu-phospho-GSK3b (5 µL) Acc-phospho-GSK3b (20 µL) GSK3b control lysate (100 µL) 5X Lysis Buffer 3 (1 mL) 10X TR-FRET Detection buffer (50 µL) 100X Phosphatase Inhibitor Cocktail (50 µL) Reactivity Human US $ 382.00 Add to cart BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of …
WebJan 31, 2024 · Whole cell lysates were immunoblotted for phospho-S536 p65, phospho-S276 p65, total p65, phospho-S176/180 IKKα/β, total IKKα/β, phospho-IκBα, total IκBα, and β-actin. (B, C) IL-2-primed NK cells were mixed with autologous target cells and stimulated with plate-immobilized aKIR and/or aPD-L1 for 1 h. Cytoplasmic and nuclear extracts ... WebWe investigated the status of serine 536 phosphorylation in human T cells and its role in activation-induced transcriptional activity. As seen in Fig. 1A, the activation of HeLa cells, but not Jurkat cells, resulted in an increase in the phosphorylation of p65 at serine 536 (henceforth referred to as phospho-p65).
WebSpecificity NF-kappaB p65 (Phospho-Ser536) Antibody detects endogenous levels of NF-kappaB p65 only when phosphorylated at serine536. Suitable treatment will increase the … WebTHUNDER™ Phosphatase Inhibitor Cocktail (100X) THUNDER™ Phospho-NF-kB (S536) + Total NF-kB TR-FRET Cell Signaling Assay Kit Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-NF-κB (S536) and Total NF-κB in cell lysates.
WebAug 12, 2024 · The protein expression of pIκB, IL-1β, IL-6, NFκB p65 (phospho S536) and TNF-α in colonic tissues was examined using Western blot analysis. Representative data are demonstrated (C) and the relative protein intensities of pIκB, IL-6, IL-1β, NFκB p65 (phospho S536) and TNF-α were normalized to GAPDH (D–H) .
WebPhospho-NF-kappaB p65 (Ser536) Antibody detects NF-kappaB p65 only when phosphorylated at serine 536. The antibody does not cross-react with the p50 subunit or other related proteins. Species Reactivity: Human, … dick grayson cryingWebTHUNDER™ Phosphatase Inhibitor Cocktail (100X) THUNDER™ Phospho-NF-kB (S536) + Total NF-kB TR-FRET Cell Signaling Assay Kit Sandwich TR-FRET immunoassay for semi … dick grayson concept artWebJan 31, 2015 · We report here that active p536 can significantly increase cell motility and transform PNT1a cells (an immortalized normal cell line), suggesting p536 plays a critical role in promoting PCa... dick grayson comic iconsWebRayBio® Phospho- NF-κB p65 (S536) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated NF-κB p65 protein in your experimental model system, you can verify pathway activation in your cell ... citizenship ceremony las vegasWebMonoclonal Antibody for studying NFkB-p65 (Ser536) phosphate. Cited in 41 publications. Validated for Western Blotting, Immunoprecipitation. Highly specific and rigorously … dick grayson crime syndicateWebThe Phospho-NFkB (Ser536) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize the protein independent of its phosphorylation state. citizenship ceremony montrealWebThis antibody is specific for human p65 (RelA) protein phosphorylated at S536. A BLAST analysis was used to suggest cross reactivity with p65 (RelA) from human, mouse and rat … dick grayson dated rocket