Ribosome half-transit times
WebbMeasurement of Ribosomal Half-Transit Time Cells were pulsed with [35S] L-methionine/ L-cystine for various times and lysed with polysome lysis buffer. 100 µl lysates were … http://genesdev.cshlp.org/content/19/18/2199.full
Ribosome half-transit times
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Webb22 juni 2010 · Based on timing of appearance of luciferase activity in kinetics assays with G-Triple and monocistronic RLuc or FLuc RNAs, we estimated ribosome transit times at 5 min for the RLuc coding region and 23 min for the whole Triple ORF (data not shown). WebbAverage ribosome half-transit times were determined by measuring the kinetics of transfer of labeled polypeptides from polysomal-bound to released polypeptides. The average …
Webbför 14 timmar sedan · Billions of dollars in new taxes and fees are on the table at the Minnesota Legislature this year.. The big picture: As they debate how to spend a historic $17.5 billion surplus, DFL legislators are looking at increases to fund everything from state parks to paid family leave. Proposed hikes would hit fishing permits, car tabs, local sales … Webb1 nov. 2007 · Increased ribosome half-transit time and increased resistance of 80S particles to 200 mM KCl. (A) The ribosome half-transit time was obtained as described …
Webb10 sep. 2013 · Ribosome half–transit time measurement. Yeasts were grown in SD medium supplemented with adenine and uracil (2% glucose) at 34°C for 4 h until reached … WebbAverage Ribosome Half-transit Time-Ribosome transit time refers to the length of time needed for a ribosome, after attaching to a mRNA, to complete translation and release a …
Webb10 juni 1977 · Ribosome half-transit times for albumin and total liver protein were 1.6 to 1.7 min in isolated liver cells derived from both normal and hypophysectomized rats. …
Webb3 mars 2016 · Ribosome half-transit time determination is based on comparison of the rates of 35 S-Methionine incorporation into total (nascent and completed) and … post secondary newfoundlandWebbMeasurement of ribosome half-transit time Cells (3 × 10 7 ) were suspended in 5 mL of labeling medium (Basal Medium-Eagle [Biological Industries] supplemented with 10% … postsecondary nameWebb18 juni 2004 · The ribosomal half-transit time is an average measurement of the length of time required for a ribosome, after it becomes attached to an mRNA, to complete … postsecondary non-degreeWebbstudy protein synthesis andribosome transit times. After a 2-hr preincubation of explant tissue in the presence of a large con-centration ofnonradioactiveleucine, … post secondary non degree jobsWebbStimulatory doses of cholecystokinin (CCK) (100 pM), bombesin (10 nM), and carbachol (10 lM) increased elongation rates (measured as ribosomal half-transit time) in … post secondary newsWebbRibosomal pause refers to the queueing or stacking of ribosomes during translation of the nucleotide sequence of mRNA transcripts. These transcripts are decoded and converted … total tools online indiaWebb27 juli 2011 · When Eg5 is inhibited, delayed ribosomal half-transit times, increased polysome peaks, and higher P/M ratios were observed. Together with the association of … total tools mitchell act