Sensifast cdna synthesis kit
WebThe SensiFAST SYBR® Hi-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA … WebSensiFAST™ HRM Kit is a post-PCR analysis method of characterizing nucleic acid samples based on their DNA strand dissociation behavior, with increasing temperature, in the …
Sensifast cdna synthesis kit
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WebcDNA: the optimal amount of cDNA to use in a single PCR is dependent upon the copy number of the target gene. We suggest using 100 ng cDNA per reaction, however it may be necessary to vary this amount. To perform a two-step RT-PCR, we recommend using the SensiFAST cDNA Synthesis Kit (BIO-65053) for reverse protocols.io WebSensiFAST cDNA Synthesis Kit can be used with SensiFAST Probe and SensiFAST SYBR® Kits for fast real-time RT-qPCR without compromising on quality, giving real-time results …
WebEfficient cDNA synthesis can be accomplished by a 10-minute incubation at 45–60°C. A 50°C incubation is recommended as a general starting point. For GC-rich or structurally complex RNA templates, increasing the cDNA synthesis incubation temperatures up to 55–60 °C may improve RT-PCR results. WebApr 14, 2024 · For RT-qPCR, reverse transcription was performed using SensiFAST cDNA Synthesis Kit (BIO-65054, meridian BIOSCIENCE), and target genes were quantified by THUNDERBIRD SYBR qPCR Mix (QPS-201, TOYOBO) and Step One Real-Time PCR System (Applied Biosystems) using the primers listed in Additional file 1: Table S3.
WebMar 2, 2024 · Total RNA was extracted from the seeded macrophage cells (RNeasy Mini Kit, QIAGEN, Germantown, MD, USA) and reverse-transcribed into cDNA using the SensiFAST™ cDNA Synthesis kit (Bioline, Aust Pty. Ltd., Auburn, NSW, Australia). WebThe complementary DNA (cDNA) was synthesized using SensiFAST™ cDNA Synthesis Kit (Bioline, Nottington, UK) according to the manufacturer’s protocol. The reverse transcription polymerase chain reaction (RT-PCR) was carried out using Solg™ Taq DNA polymerase (SolGent, Daejeon, Korea). The PCR conditions were as follows: initial denaturation ...
WebSensiFAST Probe has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes. A combination of the latest …
WebSensiFAST cDNA Synthesis Kit contains a highly-pure reverse transcriptase and optimized TransAmp™ buffer system, which includes a unique blend of random hexamers and anchored oligo (dT) primers to deliver the highest quality qPCR ready cDNA. rick springfield real nameWebGenetika Science reds threadsWebSensiFAST cDNA Synthesis Kit contains a highly-pure reverse transcriptase and optimized TransAmp™ buffer system, which includes a unique blend of random hexamers and … reds thousand oaks grand rapidsWebThe SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis, which displays excellent linearity across a wide range of starting … red stick 2 mais baratoWebSensiFAST cDNA Synthesis Kit can be used with SensiFAST Probe and SensiFAST SYBR® Kits for fast real-time RT-qPCR without compromising on quality, giving real-time results … rick springfield st augustineWebThe SensiFAST cDNA Kit provides a rapid and sensitive method for first-strand cDNA synthesis, which displays excellent linearity across a wide range of starting material. This gives the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in qPCR studies. rick springfield wait for nightWebSensiFAST™ cDNA Synthesis Kit contains a highly-pure reverse transcriptase and optimised TransAmp™ buffer system, which includes a unique blend of random hexamers and anchored oligo (dT) primers to deliver the highest quality qPCR ready cDNA. reds throwback jerseys